EC Number | Cloned (Comment) | Organism |
---|---|---|
1.8.99.B1 | expressed in Escherichia coli | Saccharolobus solfataricus |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.8.99.B1 | the X-ray crystallographic structure is solved at 1.80 A resolution | Saccharolobus solfataricus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.8.99.B1 | 19930 | - |
1 * 19930, calculated from sequence | Saccharolobus solfataricus |
1.8.99.B1 | 20995 | - |
1 * 20995, light scattering and electrospray mass spectroscopy analyses | Saccharolobus solfataricus |
1.8.99.B1 | 21000 | - |
light scattering and electrospray mass spectroscopy analyses | Saccharolobus solfataricus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.8.99.B1 | protein-dithiol + oxidized acceptor | Saccharolobus solfataricus | the enzyme is involved in disulfide bond formation | protein-disulfide + reduced acceptor | - |
? | |
1.8.99.B1 | protein-dithiol + oxidized acceptor | Saccharolobus solfataricus P2 | the enzyme is involved in disulfide bond formation | protein-disulfide + reduced acceptor | - |
? |
EC Number | Organic Solvent | Comment | Organism |
---|---|---|---|
1.8.99.B1 | guanidine-HCl | [GuHCl]/2 value is 1.5 M | Saccharolobus solfataricus |
1.8.99.B1 | urea | resistant to urea presenting a [urea]/2 value of 5 M | Saccharolobus solfataricus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.8.99.B1 | Saccharolobus solfataricus | Q97Z21 | - |
- |
1.8.99.B1 | Saccharolobus solfataricus P2 | Q97Z21 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.8.99.B1 | - |
Saccharolobus solfataricus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.8.99.B1 | protein-dithiol + oxidized acceptor | the enzyme is involved in disulfide bond formation | Saccharolobus solfataricus | protein-disulfide + reduced acceptor | - |
? | |
1.8.99.B1 | protein-dithiol + oxidized acceptor | the enzyme is involved in disulfide bond formation | Saccharolobus solfataricus P2 | protein-disulfide + reduced acceptor | - |
? | |
1.8.99.B1 | [insulin]-disulfide + reduced dithiothreitol | the enzyme does not present isomerase activity | Saccharolobus solfataricus | [insulin]-dithiol + oxidized dithiothreitol | - |
? | |
1.8.99.B1 | [insulin]-disulfide + reduced dithiothreitol | the enzyme does not present isomerase activity | Saccharolobus solfataricus P2 | [insulin]-dithiol + oxidized dithiothreitol | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.8.99.B1 | monomer | 1 * 19930, calculated from sequence | Saccharolobus solfataricus |
1.8.99.B1 | monomer | 1 * 20995, light scattering and electrospray mass spectroscopy analyses | Saccharolobus solfataricus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.8.99.B1 | Sso1120 | - |
Saccharolobus solfataricus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.8.99.B1 | 90 | - |
Tm-value | Saccharolobus solfataricus |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
1.8.99.B1 | Saccharolobus solfataricus | calculated from sequence | - |
5.3 |